Lysophospholipid acyltransferases and eicosanoid biosynthesis in zebrafish myeloid cells

  • Posted on: 5 October 2015
  • By: fcoldren
TitleLysophospholipid acyltransferases and eicosanoid biosynthesis in zebrafish myeloid cells
Publication TypeJournal Article
Year of Publication2014
AuthorsZarini S, Hankin JA, Murphy RC, Gijón MA
JournalProstaglandins Other Lipid Mediat
Date Published2014 Oct
Keywords1-Acylglycerophosphocholine O-Acyltransferase, Animals, Calcimycin, Calcium Ionophores, Eicosanoids, Lysophospholipids, Models, Animal, Myeloid Cells, Spectrometry, Mass, Electrospray Ionization, Zebrafish

Eicosanoids derived from the enzymatic oxidation of arachidonic acid play important roles in a large number of physiological and pathological processes in humans. Many animal and cellular models have been used to investigate the intricate mechanisms regulating their biosynthesis and actions. Zebrafish is a widely used model to study the embryonic development of vertebrates. It expresses homologs of the key enzymes involved in eicosanoid production, and eicosanoids have been detected in extracts from adult or embryonic fish. In this study we prepared cell suspensions from kidney marrow, the main hematopoietic organ in fish. Upon stimulation with calcium ionophore, these cells produced eicosanoids including PGE2, LTB4, 5-HETE and, most abundantly, 12-HETE. They also produced small amounts of LTB5 derived from eicosapentaenoic acid. These eicosanoids were also produced in kidney marrow cells stimulated with ATP, and this production was greatly enhanced by preincubation with thimerosal, an inhibitor of arachidonate reacylation into phospholipids. Microsomes from these cells exhibited acyltransferase activities consistent with expression of MBOAT5/LPCAT3 and MBOAT7/LPIAT1, the main arachidonoyl-CoA:lysophospholipid acyltransferases. In summary, this work introduces a new cellular model to study the regulation of eicosanoid production through a phospholipid deacylation-reacylation cycle from a well-established, versatile vertebrate model species.

Alternate JournalProstaglandins Other Lipid Mediat.
PubMed ID25175316
PubMed Central IDPMC4252513
Grant ListHL117798 / HL / NHLBI NIH HHS / United States
U54 HL117798 / HL / NHLBI NIH HHS / United States