Gene Set Gene Set Qualifier Gene Name Alternative Names Recommended Name UniProt ID NCBI Gene ID EC Species Name Taxonomy ID Pathway Information Source Evidence Type PubMed ID Notes Notes/SubPathway AAP Gold Standard HPGDS hematopoietic prostaglandin D synthase hematopoietic prostaglandin D synthase O60760 27306 5.3.99.2 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 C 21942677 COX AAP Gold Standard PTGES Microsomal prostaglandin E synthase 1 prostaglandin E synthase O14684 9536 5.3.99.3 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 E E 12672824 10377395 measured PGE2 formation COX AAP Gold Standard ACAD9 Acyl-CoA dehydrogenase family member 9, mitochondrial precursor acyl-CoA dehydrogenase family, member 9 Q9H845 28976 1.3.99.- Human 9606 Bob Murphy E 16020546 AA remodeling AAP Gold Standard ACAT1 Acetyl-CoA acetyltransferase, mitochondrial precursor acetyl-CoA acetyltransferase 1 P24752 38 Human 9606 Bob Murphy E 11294643 Uses fatty acyl-CoA and cholesterol to form cholesterol esters, may be regulated by free fatty acids AA remodeling AAP Gold Standard ACSL4 Isoform Long of Long-chain-fatty-acid--CoA ligase 4 acyl-CoA synthetase long-chain family member 4 O60488 2182 Human 9606 Bob Murphy E 21085606 21242590 ACSL4 upregulates COX2 and PG synthesis in breast cancer cell lines (PMID 21085606) and in arterial smooth muscle cells (PMID 21242590) AA remodeling AAP Gold Standard AGPAT2 Isoform 1 of 1-acyl-sn-glycerol-3-phosphate acyltransferase beta 1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase, beta) O15120 10555 Human 9606 Bob Murphy E 9242711 AA remodeling AAP Gold Standard AGPAT5 1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) Q9NUQ2 55326 Human 9606 Bob Murphy E 21173190 AA remodeling AAP Gold Standard AKR1B1 aldo-keto reductase family 1, member B1 (aldose reductase) aldo-keto reductase family 1, member B1 (aldose reductase) P15121 231 Human 9606 Tilo/Kate C E 21942677 19010934 COX AAP Gold Standard AKR1C3 aldo-keto reductase family 1, member C3 aldo-keto reductase family 1, member C3 P42330 8644 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 E 10622721 aka prostaglandin f synthase (1.1.1.188), but in UniProt this enzyme has multiple EC COX AAP Gold Standard ALOX12 arachidonate 12-lipoxygenase arachidonate 12-lipoxygenase P18054 239 1.13.11.31 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 E C E 6783111 12432922 17493578 LOX AAP Gold Standard ALOX12B arachidonate 12-lipoxygenase, 12R type arachidonate 12-lipoxygenase, 12R type O75342 242 1.13.11.- Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 C 12432922 LOX AAP Gold Standard ALOX15 arachidonate 15-lipoxygenase arachidonate 15-lipoxygenase P16050 246 1.13.11.33 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 E C 2496760 12432923 LOX AAP Gold Standard ALOX15B arachidonate 15-lipoxygenase, type B arachidonate 15-lipoxygenase, type B O15296 247 1.13.11.33 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 E C 17493578 12432923 LOX AAP Gold Standard ALOX5 arachidonate 5-lipoxygenase arachidonate 5-lipoxygenase P09917 240 1.13.11.34 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 E C 3038871 12432920 LOX AAP Gold Standard ALOX5AP Arachidonate 5-lipoxygenase-activating protein; FLAP arachidonate 5-lipoxygenase-activating protein P20292 241 Human 9606 Bob Murphy Reactome: REACT_147851.3 C E E 21936577 8440384 19233132 Interacts with 5-LO LOX AAP Gold Standard ALOXE3 Epidermis-type lipoxygenase 3 arachidonate lipoxygenase 3 Q9BYJ1 59344 Human 9606 Bob Murphy C E E E 21936577 20923767 12881489 20921226 In 12881489: Lack of LOX activity for substrates: linoleic, arachidonic, and eicosapentaenoic acids, the methyl esters of arachidonic acid and linoleic acid, arachidonyl phosphatidylcholine, anandamide, and the cholesteryl ester of arachidonic acid. Does use 12R-HpETE, 12S-HPETE, 15S-HPETE, 8R-HPETE, and the 9S-, 13R-, and 13S-hydroperoxides of linoleic acid as substrates but preferes the 12R substrate. Some kinetic information is available. LOX AAP Gold Standard CBR1 carbonyl reductase 1 carbonyl reductase 1 P16152 873 1.1.1.184 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 E 7005231 6586494 18493841 COX AAP Gold Standard CYP1A1 P04798 1543 Human 9606 arachidonic acid metabolism Reactome: REACT_147851.3 E 15258110 metabolites are broken down into breakdown into % produced AAP Gold Standard CYP1A2 cytochrome P450 1A2 cytochrome P450, family 1, subfamily A, polypeptide 2 P05177 1544 1.14.14.1 Human 9606 celecoxib pathway PharmGKB: PA165816736 Reactome: REACT_147851.3 E E 7625847 15258110 PMID 7625847: Characterizes AA metabolism by several human CYPs, PMID 15258110: Characterizes AA metabolism by CYP1A1, CYP1A2 and CYP1B1 cytochrome P450,Celecoxib metabolism AAP Gold Standard CYP1B1 Q16678 1545 Human 9606 arachidonic acid metabolism Reactome: REACT_147851.3 E 15258110 metabolites are broken down into breakdown into % produced AAP Gold Standard CYP2B6 cytochrome P450, family 2, subfamily B, polypeptide 6 cytochrome P450, family 2, subfamily B, polypeptide 6 P20813 1555 1.14.14.1 Human 9606 arachidonic acid metabolism KEGG: hsa00590 P C 21116621 11773611 (21116621 )Phylogenetic analysis of all CYP2 family members, may be useful when curating non-human CYP enzymes. 11773611 cites articles that when inucbated with AA produces EETS cytochrome P450 AAP Gold Standard CYP2C19 cytochrome P450, family 2, subfamily C, polypeptide 19 cytochrome P450, family 2, subfamily C, polypeptide 19 P33261 1557 1.14.14.1 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 E C 9435160 11773611 cytochrome P450 AAP Gold Standard CYP2C8 cytochrome P450, family 2, subfamily C, polypeptide 8 cytochrome P450, family 2, subfamily C, polypeptide 8 P10632 1558 1.14.14.1 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 E C 9435160 11773611 cytochrome P450 AAP Gold Standard CYP2C9 cytochrome P450, family 2, subfamily C, polypeptide 9 cytochrome P450, family 2, subfamily C, polypeptide 9 P11712 1559 1.14.14.1 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 E C 9435160 11773611 cytochrome P450 AAP Gold Standard CYP2D6 cytochrome P450 2D6 cytochrome P450, family 2, subfamily D, polypeptide 6 P10635 1565 Human 9606 celecoxib pathway PharmGKB: PA165816736 E 12891223 18206980 CYP2D6 is inhibited by celecoxib, but is not directly involved in arachidonic acid metabolism. In 18206980, metabolizes AA Celecoxib metabolism AAP Gold Standard CYP2E1 cytochrome P450, family 2, subfamily E, polypeptide 1 cytochrome P450, family 2, subfamily E, polypeptide 1 P05181 1571 1.14.14.1 Human 9606 arachidonic acid metabolism KEGG: hsa00590 E 18206980 Can metabolize AA but had higher rates for other substrates cytochrome P450 AAP Gold Standard CYP2J2 cytochrome P450, family 2, subfamily J, polypeptide 2 cytochrome P450, family 2, subfamily J, polypeptide 2 P51589 1573 1.14.14.1 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 E C 8631948 11773611 cytochrome P450 AAP Gold Standard CYP2U1 cytochrome P450, family 2, subfamily U, polypeptide 1 cytochrome P450, family 2, subfamily U, polypeptide 1 Q7Z449 113612 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 E 14660610 cytochrome P450 AAP Gold Standard CYP3A4 cytochrome P450 3A4 cytochrome P450, family 3, subfamily A, polypeptide 4 P08684 1576 Human 9606 celecoxib pathway PharmGKB: PA165816736 E C E 9435160 11773611 10773015 CYP3A4 plays a minor role in both arachidonic acid metabolism and celecoxib metabolism Celecoxib metabolism AAP Gold Standard CYP4A11 cytochrome P450, family 4, subfamily A, polypeptide 11 cytochrome P450, family 4, subfamily A, polypeptide 11 Q02928 1579 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 Reactome: REACT_13645.1 E 10660572 15611369 In 15611369, catalyzed the metabolism of AA to 20- and 19-HETE cytochrome P450 AAP Gold Standard CYP4F11 cytochrome P450, family 4, subfamily F, polypeptide 11 cytochrome P450, family 4, subfamily F, polypeptide 11 Q9HBI6 57834 1.14.13.30 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 Reactome: REACT_13645.1 E E 19932081 15364545 Purified from heterologous expression in E. coli; assayed in yeast microsomes (kinetic info here) cytochrome P450 AAP Gold Standard CYP4F2 cytochrome P450, family 4, subfamily F, polypeptide 2 cytochrome P450, family 4, subfamily F, polypeptide 2 P78329 8529 1.14.13.30 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 Reactome: REACT_13645.1 E E C C 2986111 4044832 17786636 11773611 cytochrome P450 AAP Gold Standard CYP4F22 cytochrome P450, family 4, subfamily F, polypeptide 22 cytochrome P450, family 4, subfamily F, polypeptide 22 Q6NT55 126410 1.14.13.30 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 Reactome: REACT_13645.1 E 19919823 oxygenated 20:4n−6 at C-18 cytochrome P450 AAP Gold Standard CYP4F3 cytochrome P450, family 4, subfamily F, polypeptide 3 cytochrome P450, family 4, subfamily F, polypeptide 3 Q08477 4051 1.14.13.30 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 Reactome: REACT_13645.1 E 2986111 4044832 2836406 cytochrome P450 AAP Gold Standard CYP4F8 cytochrome P450, family 4, subfamily F, polypeptide 8 cytochrome P450, family 4, subfamily F, polypeptide 8 P98187 11283 1.14.13.30 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 Reactome: REACT_13645.1 C 11773611 cytochrome P450 AAP Gold Standard CYSLTR1 Cysteinyl leukotriene receptor 1 cysteinyl leukotriene receptor 1 Q9Y271 10800 Human 9606 Bob Murphy Reactome: REACT_18352.1 C 21936577 Look up primary ref LOX AAP Gold Standard CYSLTR2 cysteinyl leukotriene receptor 2 cysteinyl leukotriene receptor 2 Q9NS75 57105 Human 9606 Literature Reactome: REACT_18352.1 C 21936577 Look up primary ref LOX AAP Gold Standard DPEP1 MBD1 Dipeptidase 1 P16444 1800 Human 9606 Bob Murphy Reactome: REACT_147851.3 E 2768222 This gene was added based on the important function of catalysis of LTD4 to LTE4. This function was suggested by Bob Murphy. Experimental reference is for dipeptidase isolated from kidney. Crystal structure 12144777. MAY BE REFERRED TO AS MBD 1 AAP Gold Standard DPEP2 MBD2 Dipeptidase 2 Q9H4A9 64174 Human 9606 Bob Murphy Reactome: REACT_147851.3 E E 3563417 6293969 This gene was added based on the important function of catalysis of LTD4 to LTE4. This function was suggested by Bob Murphy. By similarity in UniProt. The experimental references are for dipeptidase not isolated from kidney. MAY BE REFERRED TO AS MBD 2 AAP Gold Standard EPHX2 epoxide hydrolase 2, cytoplasmic epoxide hydrolase 2, cytoplasmic P34913 2053 3.3.2.10 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 E 12468260 cytochrome P450 AAP Gold Standard GGT1 gamma-glutamyltransferase 1 gamma-glutamyltransferase 1 P19440 2678 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 E 21447318 Kinetic analysis of GGT1 and GGT5 using LTC4 as a substrate, includes kinetic information LOX AAP Gold Standard GGT5 gamma-glutamyltransferase 5 gamma-glutamyltransferase 5 P36269 2687 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 E 21447318 Kinetic analysis of GGT1 and GGT5 using LTC4 as a substrate, includes kinetic information LOX AAP Gold Standard GPX1 glutathione peroxidase 1 glutathione peroxidase 1 P07203 2876 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 E 10880344 GPX1 is the main inhibitor of 5-LO activity in monocytic cells in vitro. Inhibition is not due to protein-protein interactions, but likely due to the ability of GPxs to scavenge peroxides (i.e. HPETE) LOX AAP Gold Standard GPX3 glutathione peroxidase 3 (plasma) glutathione peroxidase 3 (plasma) P22352 2878 Human 9606 arachidonic acid metabolism KEGG: hsa00590 E 3693360 8373192 GPX3 can reduce lipid and phosholipid hydroperoxides, including 15-HPETE. The ability to inhibit the LOX pathway has not been specifically characterized. LOX AAP Gold Standard GPX4 glutathione peroxidase 4, phospholipid hypdroperoxide glutathione peroxidase Phospholipid hydroperoxide glutathione peroxidase, mitochondrial P36969 2879 1.11.1.12 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 E P E E 8954158 10549853 6816802 11115402 GPX4 inhibits 5-LO activity in B-lymphocytic cell lines in vitro. This is presumably by scavenging peroxides (i.e. HPETE), but the specific reaction has not been assayed. 5S-HpETE to 5S-HETE inferred from rabbit (PMID:10549853). Human platelets assay provides (what I would consider) indirect evidence of the 5S-HpETE to 5S-HETE (PMID: 6816802), although may support the 12S-HpETE rxn. In (11115402) evidence supports a role in 12S-HpETE rxn and activity with PAPC-OOH as a substrate. LOX AAP Gold Standard HPGD P15428 3248 Human 9606 arachidonic acid metabolism Reactome: REACT_147851.3 E P E 10837478 3954355 16828555 LXA4 to 15k-LXA4 in humans(10837478). PGD2/E2/F2a rxn (3954355) is inferred from rabbits. Human enzyme assayed with PGE2 in (16828555) AAP Gold Standard LCAT Phosphatidylcholine-sterol acyltransferase precursor lecithin-cholesterol acyltransferase P04180 3931 2.3.1.43 Human 9606 Bob Murphy C E 11590208 23023370 Experimental: total plasma levels of AA were gene-dependently decreased in carriers of LCAT mutations AA remodeling AAP Gold Standard LIPG Isoform 1 of Endothelial lipase precursor lipase, endothelial Q9Y5X9 9388 Human 9606 Bob Murphy E E 14622025 15834125 hydrolysis of PAPC in spherical rHDL (mentioned that recombinant EL is used, context of article suggests it is human) AA remodeling AAP Gold Standard LPCAT2 Isoform 1 of Lysophosphatidylcholine acyltransferase 2 lysophosphatidylcholine acyltransferase 2 Q7L5N7 54947 Human 9606 Bob Murphy E 20363836 AA remodeling AAP Gold Standard LPCAT3 Lysophosphatidylcholine acyltransferase; MBOAT5 lysophosphatidylcholine acyltransferase 3 Q6P1A2 10162 Human 9606 Bob Murphy E E E 18772128 18781350 19351971 LPCAT3 is (cDNA) NM_005768 (protein) NP_005759. Assayed with Arachidonoyl-CoA AA remodeling AAP Gold Standard LTA4H leukotriene A4 hydrolase leukotriene A4 hydrolase P09960 4048 3.3.2.6 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 E 3038871 8157657 LOX AAP Gold Standard LTB4R Leukotriene B4 receptor 1; BLT1 leukotriene B4 receptor Q15722 1241 Human 9606 Bob Murphy Reactome: REACT_18352.1 E 9177352 specific LTB4 binding, with a Kd 0.154nM LOX AAP Gold Standard LTB4R2 Leukotriene B4 receptor 2; BLT2 leukotriene B4 receptor 2 Q9NPC1 56413 Human 9606 Bob Murphy Reactome: REACT_18352.1 E E 11278893 10934230 binds LTB4 with Kd 23 nM LOX AAP Gold Standard LTC4S leukotriene C4 synthase leukotriene C4 synthase Q16873 4056 4.4.1.20 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 C 12432940 LOX AAP Gold Standard MAPKAPK2 P49137 9261 Human 9606 arachidonic acid metabolism Reactome: REACT_147851.3 E E 10779545 11844797 AAP Gold Standard MBOAT7 membrane bound O-acyltransferase domain containing, LPIAT membrane bound O-acyltransferase domain containing 7 Q96N66 79143 Human 9606 Bob Murphy E 18772128 Arachidonoyl-CoA as substrate AA remodeling AAP Gold Standard PLA2G10 phospholipase A2, group X phospholipase A2, group X O15496 8399 Human 9606 arachidonic acid metabolism KEGG: hsa00590 E 12359733 17148553 16122976 15611272 Exhibits calcium-dependent phospholipase activity. Appears to exhibit a preference for AA over other phospholipids. PMID 15611272 also provides indirect evidence that PLA2G10 may induce cytokine release in human lung macrophages via activation of the M-type sPLA2 receptor AA remodeling AAP Gold Standard PLA2G12A phospholipase A2, group XIIA phospholipase A2, group XIIA Q9BZM1 81579 Human 9606 arachidonic acid metabolism KEGG: hsa00590 E 11031251 12522102 PMID 11031251: mRNA expressed in heart, skeletal muscle and kidney, exhibits calcium-dependent phosholipase activity in lipid vesicles (did not specifically test ability to liberate AA) PMID 12522102: activity of PLA2G12 appeared to be quite low compared to other PLAs AA remodeling AAP Gold Standard PLA2G1B phospholipase A2, group IB (pancreas) phospholipase A2, group IB (pancreas) P04054 5319 3.1.1.4 Human 9606 arachidonic acid metabolism KEGG: hsa00590 E 12359733 16005851 15611272 PMID 12359733: Exhibits phospholipase activity PMID 16005851: Induced the concentration-dependent production of LTB4, but this was not due to AA release or phospholipase activity. May be due to activation of M-type sPLA2 receptor, but this was not determined in the study. PMID 15611272 provides indirect evidence that PLA2G1B induces cytokine production in human lung macrophages via activation of the M-type receptor AA remodeling AAP Gold Standard PLA2G2A phospholipase A2, group IIA (platelets, synovial fluid) phospholipase A2, group IIA (platelets, synovial fluid) P14555 5320 Human 9606 arachidonic acid metabolism KEGG: hsa00590 E 2925608 11786516 PMID 2925608: Isolation, cloning, and activity characterization of PLA2G2A from rheumatoid arthritis synovial fluid, PMID 11786516: Type IIA sPLA2 is pro-inflammatory and induces COX2 expression in monocytic cells AA remodeling AAP Gold Standard PLA2G2D phospholipase A2, group IID phospholipase A2, group IID Q9UNK4 26279 Human 9606 arachidonic acid metabolism KEGG: hsa00590 E 10455175 12359733 hydrolyzes phospolipids, but not preferentially for arachidonic acid AA remodeling AAP Gold Standard PLA2G2E phospholipase A2, group IIE phospholipase A2, group IIE Q9NZK7 30814 Human 9606 arachidonic acid metabolism KEGG: hsa00590 E 10681567 12359733 hydrolyzes phospolipids, but not preferentially for arachidonic acid AA remodeling AAP Gold Standard PLA2G2F phospholipase A2, group IIF phospholipase A2, group IIF Q9BZM2 64600 Human 9606 arachidonic acid metabolism KEGG: hsa00590 E 12359733 11112443 PMID 12359733 - Exhibits calcium-dependent phospholipase activity, with slight preference for AA, PMID 11112443 - mRNA highly expressed in liver, kidney, placenta, thymus, and testis (also looked at tissue distribution of other PLAs), demonstrated general phospholipase activity with palmitoyl substrates, but did not hydrolysis of arachidonic acid from phospholipid AA remodeling AAP Gold Standard PLA2G3 phospholipase A2, group III phospholipase A2, group III Q9NZ20 50487 Human 9606 arachidonic acid metabolism KEGG: hsa00590 E 12522102 sPLA-III was expressed in multiple human cell lines and exhibited phosholipase activity in expressed enzyme systems and in cell culture, no preference for AA over other phospholipids AA remodeling AAP Gold Standard PLA2G4A phospholipase A2, group IVA (cytosolic, calcium-dependent) phospholipase A2, group IVA (cytosolic, calcium-dependent) P47712 5321 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 E 21247147 1904318 PMID 21247147 characterizes the enzymatic function of mutant enzyme discovered in a cPLA2-deficient patient, PMID 1904318 describes the initially cloning an enzymatic characterization of cPLA2 - preferentially releases AA from sn-2 position of phospholipids - only PLA with strong AA preference AA remodeling AAP Gold Standard PLA2G4B phospholipase A2, group IVB (cytosolic) phospholipase A2, group IVB (cytosolic) P0C869 100137049 Human 9606 arachidonic acid metabolism KEGG: hsa00590 E 10358058 Exhibits calcium-dependent phospholipase activity, with preference from sn-1 position, mRNA detected in a wide range of tissues AA remodeling AAP Gold Standard PLA2G4C phospholipase A2, group IVC (cytosolic, calcium-independent) phospholipase A2, group IVC (cytosolic, calcium-independent) Q9UP65 8605 Human 9606 arachidonic acid metabolism KEGG: hsa00590 E 9705332 Exhibits calcium-independent phospholipase activity with equal affinity for the sn-1 and sn-2 positions, mRNA highly expressed in skeletal muscle and heart, with lower levels in spleen, placenta, brain and pancreas AA remodeling AAP Gold Standard PLA2G4D phospholipase A2, group IVD (cytosolic) phospholipase A2, group IVD (cytosolic) Q86XP0 283748 3.1.1.4 Human 9606 arachidonic acid metabolism KEGG: hsa00590 E 14709560 mRNA expression is upregulated in skin from patients with psoriasis or atopic dermatitis and is expressed in skin, cervix and prostate, exhibit calcium-dependent phospholipase activity with a preference for linoleic, rather than arachidonic, acid at the sn-2 position AA remodeling AAP Gold Standard PLA2G5 phospholipase A2, group V phospholipase A2, group V P39877 5322 Human 9606 arachidonic acid metabolism KEGG: hsa00590 E 8300559 9767110 mRNA detected in lung, heart, and placenta, exhibits calcium-dependent phospholipase activity AA remodeling AAP Gold Standard PLA2G6 phospholipase A2, group VI (cytosolic, calcium-independent); iPLA2 phospholipase A2, group VI (cytosolic, calcium-independent) O60733 8398 Human 9606 arachidonic acid metabolism KEGG: hsa00590 E C 9417066 16973413 Exhibits calcium-independent phospholipase activity, mRNA detected in B-cell lines AA remodeling AAP Gold Standard PLB1 phospholipase B1 phospholipase B1 Q6P1J6 151056 3.1.1.5 Human 9606 arachidonic acid metabolism KEGG: hsa00590 E 11927584 AA remodeling AAP Gold Standard PTGDR DP1 prostaglandin D2 receptor (DP) Q13258 5729 Human 9606 Tilo/Kate Reactome: REACT_18352.1 C 21752876 Review article suggests evidence COX AAP Gold Standard PTGDR2 DP2 prostaglandin D2 receptor 2 Q9Y5Y4 11251 Human 9606 Tilo/Kate Reactome: REACT_18352.1 C 21752876 Review article suggests evidence COX AAP Gold Standard PTGDS Lipocalin-type prostaglandin-D synthase prostaglandin D2 synthase 21kDa (brain) P41222 5730 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 C E 21942677 20667974 COX AAP Gold Standard PTGER1 prostaglandin E receptor 1 prostaglandin E receptor 1 (subtype EP1), 42kDa P34995 5731 Human 9606 Tilo/Kate Reactome: REACT_18352.1 C 21752876 Review article suggests evidence COX AAP Gold Standard PTGER2 prostaglandin E receptor 2 prostaglandin E receptor 2 (subtype EP2), 53kDa P43116 5732 Human 9606 Tilo/Kate Reactome: REACT_18352.1 C 21752876 Review article suggests evidence COX AAP Gold Standard PTGER3 prostaglandin E receptor 3 (subtype EP3) prostaglandin E receptor 3 (subtype EP3) P43115 5733 Human 9606 Tilo/Kate Reactome: REACT_18352.1 C 21752876 Review article suggests evidence COX AAP Gold Standard PTGER4 prostaglandin E receptor 4 (subtype EP4) prostaglandin E receptor 4 (subtype EP4) P35408 5734 Human 9606 Tilo/Kate Reactome: REACT_18352.1 C 21752876 Review article suggests evidence COX AAP Gold Standard PTGES2 Microsomal prostaglandin E synthase 2 prostaglandin E synthase 2 Q9H7Z7 80142 Human 9606 arachidonic acid metabolism KEGG: hsa00590 E C 12804604 21942677 COX AAP Gold Standard PTGES3 cytosolic prostaglandin E synthase 3 prostaglandin E synthase 3 (cytosolic) Q15185 10728 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 E C 10922363 21942677 COX AAP Gold Standard PTGFR prostaglandin F receptor (FP) prostaglandin F receptor (FP) P43088 5737 Human 9606 Tilo/Kate Reactome: REACT_18352.1 C 21752876 Review article suggests evidence COX AAP Gold Standard PTGIR prostaglandin I2 (prostacyclin) receptor (IP) prostaglandin I2 (prostacyclin) receptor (IP) P43119 5739 Human 9606 Tilo/Kate Reactome: REACT_18352.1 C 21752876 Review article suggests evidence COX AAP Gold Standard PTGIS prostaglandin I2 (prostacyclin) synthase prostaglandin I2 (prostacyclin) synthase Q16647 5740 5.3.99.4 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 Reactome: REACT_13645.1 C 21942677 COX AAP Gold Standard PTGR1 Q14914 22949 Human 9606 arachidonic acid metabolism Reactome: REACT_147851.3 P E P E 17449869 8576264 8394361 10837478 Rxn with 15k-PGE2/F2a as substrate is inferred from mice (17449869). For rxn with LTB4 as substrate (8576264) human cDNA is cloned (there are a few citations that suggest activity may have been assayed for the human enzyme), and activity is inferred from pig (8394361). Some assays were carried out with human enzyme in (10837478) which is associated with the 15k-LXA4 substrate in Reactome. AAP Gold Standard PTGS1 prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase) prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase) P23219 5742 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 Reactome: REACT_1396.3 C C 18952571 21942677 COX AAP Gold Standard PTGS2 prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) P35354 5743 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 E C 16373578 21942677 COX AAP Gold Standard TBXA2R thromboxane A2 receptor thromboxane A2 receptor P21731 6915 Human 9606 Tilo/Kate Reactome: REACT_18352.1 C 21752876 Review article suggests evidence COX AAP Gold Standard TBXAS1 thromboxane A synthase 1 (platelet) thromboxane A synthase 1 (platelet) P24557 6916 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 Reactome: REACT_13645.1 C E E 21942677 7925341 11465543 7925341 and 11465543 from Reactome COX AAP Gold Standard ABCC1 Multi-drug resistance-associated protein 1 (MRP-1) ATP-binding cassette sub-family C member 1, LTC4 transporter P33527 4363 Human 9606 Reactome: REACT_147851.2 Keyword search E 7961706 8621643 PMID 7961706: Characterized the ability of MRP-1 to transport various cysLTs in membrane vesicles from transfected HeLa cells, transported LTC4 most efficiently, then LTD4, then LTE4, PMID 8621643: Further characterized LTC4 transport in membrane vesicles and tested other potential substrates (mostly chemotherapeutic agents) LOX AAP Gold Standard ABCC4 Multi-drug resistance-associated protein 4 (MRP-4) ATP-binding cassette sub-family C member 4 O15439 10257 Human 9606 Review article: PMID 21936577 E 17959747 Characterized the ability of ABCC4 to transport LTB4, LTC4, and LTD4 in multiple cell types/lines, transported LTB4 in a GSH-dependent manner, transported LTC4 in a GSH-independent manner, transport of LTD4 was increased in the presence of GSH, also characterized the kinetics of transport LOX AAP Gold Standard PNPLA2 iPLA2zeta, TTS-2.2 Q96AD5 57104 Human 9606 literature E 15364929 PMID 15364929 shows that the human protein is capable of releasing AA from a triacylglycerol in vitro AAP Gold Standard ACSL6 FACL6, LACS5 Q9UKU0 23305 Human 9606 Jens/Ravi E C 10548543 15292367 PMID 10548543 demonstrates that it can form arachidonyl-CoA. AAP Gold Standard PLA2G16 H-rev107 P53816 11145 Human 9606 Jens/Ravi E 19615464 When assayed with radioactive substrates with palmitoyl at the sn-1 position and arachidonoyl at the sn-2 position more PLA1 activity than PLA2 activity is observed (19615464). AAP Gold Standard FADS2 delta6 desaturase Fatty acid desaturase 2 O95864 9415 1.14.19.- Human 9606 Bob Murphy E 12713571 11290821 With [14]C-linoleate as substrate for CHO transfected with human gene dihomo-gamma-linolenate was detected (12713571). A human skin fibroblast with delta6-desaturase deficiency (by Northern blot) converted 85-95% less [1-14C]linoleic acid to arachidonic acid (11290821). Linoleic acid elongation AAP Gold Standard FADS1 delta5 desaturase Fatty acid desaturase 1 O60427 3992 1.14.19.- Human 9606 Bob Murphy E 10601301 10769175 CHO expressing human gene converted 20:3(n-6) to 20:4(n-6) while nontransformed cells displayed no increase in total cellular content of 20:4(n-6) (PMID:10601301). Mouse fibroblast cells expressing the human gene converted 20:3(n-6) to arachidonic acid (PMID:10769175). Linoleic acid elongation AAP Gold Standard ELOVL5 Elongation of very long chain fatty acids protein 5 ELOVL fatty acid elongase 5 Q9NYP7 60481 2.3.1.199 Human 9606 Bob Murphy E 10970790 20937905 Yeast expressing the human gene grown in media containing gamma-linolenic acid converted it to dihomo-gamma-linolenic acid (PMID:10970790). Total membrane fraction prepared from HEK 293T cells overproducing ELOV5, and a mixture containing [14C]malonyl-CoA and C18:3(n-6)-CoA elongated gamma-linolenic acid. Figure 1D suggests the elongation stoped at 20:3(n-6) (PMID:20937905). Linoleic acid elongation AAP Gold Standard ELOVL2 Elongation of very long chain fatty acids protein 2 ELOVL fatty acid elongase 2 Q9NXB9 54898 2.3.1.199 Human 9606 Bob Murphy E 20937905 12371743 Total membrane fraction prepared from HEK 293T cells overproducing ELOV2, and a mixture containing [14C]malonyl-CoA and C18:3(n-6)-CoA did not result in elongation. Elongation occurred when the reaction mixture contained C20:4-CoA (PMID:20937905). 22:4(n-6) and 24:4(n-6) were detected from yeast expressing the human gene elongated and incubated with 20:4(n-6). No elongated fatty acids were detected when incubating with 18:3(n-6). Linoleic acid elongation AAP Likely LIPA Isoform 1 of Lysosomal acid lipase/cholesteryl ester hydrolase precursor lipase A, lysosomal acid, cholesterol esterase P38571 3988 Human 9606 Bob Murphy E 8112342 activity assayed with oleoyl- substrates AA remodeling AAP Likely LPCAT1 1-acylglycerophosphocholine O-acyltransferase 1; AYTL2 lysophosphatidylcholine acyltransferase 1 Q8NF37 79888 Human 9606 Bob Murphy E 19383981 Tissue distribution is given, enzyme activity assay done with palmitoyl-LPC and palmitoyl-CoA. Thus, no AA substrates AA remodeling AAP Likely PAFAH2 Platelet-activating factor acetylhydrolase 2, cytoplasmic platelet-activating factor acetylhydrolase 2, 40kDa Q99487 5051 Human 9606 Bob Murphy E 11294621 Activity assayed did not see a substrate that would lead to AA release AA remodeling AAP Likely SLC27A2 Very-long-chain acyl-CoA synthetase solute carrier family 27 (fatty acid transporter), member 2 O14975 11001 Human 9606 Bob Murphy E 22578083 assayed with C16:0 AA remodeling AAP Likely SOAT1 Sterol O-acyltransferase 1 sterol O-acyltransferase 1 P35610 6646 Human 9606 Bob Murphy E 8407899 also known as acyl-Coenzyme A:cholesterol acyltransferase 1 (ACAT) from 21239516. There is an ACAT1, which has a different gene ID. How will we keep them clear? 8407899 oleate assay AA remodeling AAP Likely ACSL3 Long-chain-fatty-acid--CoA ligase 3 acyl-CoA synthetase long-chain family member 3 O95573 2181 6.2.1.3 Human 9606 Bob Murphy E E 17762044 17379924 Assayed with several substrates but not AA AA remodeling AAP Likely AWAT2 DGAT2L4 acyl-CoA wax alcohol acyltransferase 2 Q6E213 158835 Human 9606 Bob Murphy E E 16106050 15671038 (16106050) does not assay activity for arachidonyl-CoA. Does assay for palmitoyl-CoA. (15671038) assays for arachidyl C-20 alcohol, but not arachidonyl-CoA AA remodeling AAP Likely GPX2 glutathione peroxidase 2 (gastrointestinal) glutathione peroxidase 2 (gastrointestinal) P18283 2877 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 P E 10549853 8428933 That the human enzyme carries out reaction 5-HPETE to 5-HETE is predicted from an experiment with the GPX in rabbit. Experimental evidence demonstrates presence of enzyme in humans. The human enzyme can reduce linoleic acid hydroperoxide, but the activity for the conversion of HPETE to HETE has not been determined. LOX AAP Predicted CYP4A22 cytochrome P450, family 4, subfamily A, polypeptide 22 cytochrome P450, family 4, subfamily A, polypeptide 22 Q5TCH4 284541 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 Reactome: REACT_13645.1 C E 17786636 15611369 More references could be checked from the review. In (15611369) did not generate a typical reduced P450 CO-bound complex and did not catalyzed the metabolism of AA to 20- and 19-HETE. UniProt states no activity with AA or prostaglandin A1 cytochrome P450 AAP Predicted ACSL1 Isoform 1 of Long-chain-fatty-acid--CoA ligase 1 acyl-CoA synthetase long-chain family member 1 P33121 2180 Human 9606 Bob Murphy C E P 15292367 20176858 23153419 PMID 15292367 provides nomenclature for mammalian ACS, may be useful for future curation, PMID 20176858 describes a genetic association study between ACSL1 SNPs and metabolic syndrome, It appears that metabolic activity has largely been predicted from mouse and rat functional studies, as I was unable to find a primary reference for human ACSL1 activity (KNT) AA remodeling AAP Predicted CYP2C18 cytochrome P450, family 2, subfamily C, polypeptide 18 cytochrome P450, family 2, subfamily C, polypeptide 18 P33260 1562 1.14.14.1 Human 9606 arachidonic acid metabolism KEGG: hsa00590 In PharmGKB involved in metabolism of R-warfarin. (http://www.pharmgkb.org/pathway/PA145011113#). Did not find in BRENDA nor too much info in UniProt. Could not find a relevant publication. No evidence found, but listed in KEGG. cytochrome P450 AAP Predicted LIPE Isoform 1 of Hormone-sensitive lipase lipase, hormone-sensitive Q05469 3991 Human 9606 Bob Murphy E C 9359844 14725507 7240206 activity in rat, but not with arachidonyl-. Experimental article demonstrates tissue distribution. Did not yet locate hydrolysis of arachidonyl substrate (FC) AA remodeling AAP Predicted PLA2G12B phospholipase A2, group XIIB phospholipase A2, group XIIB Q9BX93 84647 3.1.1.4 Human 9606 arachidonic acid metabolism KEGG: hsa00590 E C C C C 14516201 15253885 16973413 18931897 12432908 PMID 14516201 - Protein was cloned, but did not exhibit any phospholipase activity, mRNA expressed in liver, kidney, skeletal muscle, and heart AA remodeling AAP Predicted CBR3 carbonyl reductase 3 carbonyl reductase 3 O75828 874 1.1.1.184 Human 9606 arachidonic acid metabolism KEGG: hsa00590 E E 18493841 19841672 In (18493841) No traceable activity with PGA1, PGE2, PGA1-GSH, PGE2-GSH as substrates. In (19841672) author states, "CBR3 shows no activity towards eicosanoids." COX AAP Predicted FAM213B family with sequence similarity 213, member B family with sequence similarity 213, member B Q8TBF2 127281 Human 9606 arachidonic acid metabolism KEGG: hsa00590 Reactome: REACT_147851.3 P 18006499 20950588 inferred from mice COX AAP Predicted GGT6 gamma-glutamyltransferase 6 gamma-glutamyltransferase 6 Q6P531 124975 Human 9606 arachidonic acid metabolism KEGG: hsa00590 P 18357469 Enzymatic activity has not been categorized, but has high level of homology with GGT1 and GGT5. cDNA is present in EST database, but is limited. LOX AAP Predicted GGT7 gamma-glutamyltransferase 7 gamma-glutamyltransferase 7 Q9UJ14 2686 Human 9606 arachidonic acid metabolism KEGG: hsa00590 P 18357469 Enzymatic activity has not been categorized, but has high level of homology with GGT1 and GGT5 LOX AAP Predicted GPX5 glutathione peroxidase 5 (epididymal androgen-related protein) glutathione peroxidase 5 (epididymal androgen-related protein) O75715 2880 Human 9606 arachidonic acid metabolism KEGG: hsa00590 P 9639555 Initially identified by homology with rat and monkey GPx5. In human epididymis, the majority of transcripts contain a splicing error. Protein was not detected, but may be expressed in low amounts. Enzymatic function has not been characterized. LOX AAP Predicted GPX6 glutathione peroxidase 6 (olfactory) glutathione peroxidase 6 (olfactory) P59796 257202 Human 9606 arachidonic acid metabolism KEGG: hsa00590 P 12775843 Gene has been discovered, but enzymatic activity has not been characterized LOX AAP Predicted GPX7 glutathione peroxidase 7 glutathione peroxidase 7 Q96SL4 2882 Human 9606 arachidonic acid metabolism KEGG: hsa00590 P 15294905 Protein was identified and peroxidase activity was assayed in human breast cancer cell lines. AA sequence is ~40-45% homologous to GPX1 and GPX4. The role of GPX7 (aka NPGPx) in healthy tissue has not been determined. COX AAP Predicted PLA2G4E phospholipase A2, group IVE phospholipase A2, group IVE Q3MJ16 123745 Human 9606 arachidonic acid metabolism KEGG: hsa00590 P 16814865 15866882 Identified by homology with mouse enzyme (which exhibits phospholipase activity per PMID:15866882), but has not been cloned and activity has not been determined. AA remodeling AAP Predicted PLA2G4F phospholipase A2, group IVF phospholipase A2, group IVF Q68DD2 255189 Human 9606 arachidonic acid metabolism KEGG: hsa00590 P 16814865 15866882 Identified by homology with mouse enzyme (which exhibits phospholipase activity per PMID:15866882), but has not been cloned and activity has not been determined. AA remodeling AAP Predicted AGPAT4 1-acyl-sn-glycerol-3-phosphate acyltransferase delta 1-acylglycerol-3-phosphate O-acyltransferase 4 (lysophosphatidic acid acyltransferase, delta) Q9NRZ5 56895 Human 9606 Bob Murphy P C 15367102 18718904 (15367102) states that this isoform has been described, and a few references are provided. NP_064518 from (18718904). Function is "By similarity" in UniProt. No papers assaying enzyme activity found yet. Review may be helpful, but also presents no enzyme characterization. AA remodeling AAP Predicted ACSL5 Isoform Long of Long-chain-fatty-acid--CoA ligase 5 acyl-CoA synthetase long-chain family member 5 Q9ULC5 51703 Human 9606 Bob Murphy P E 20798351 19880178 Activity appears to be predicted based on rodent enzymes (PMID 20798351), PMID 19880178: indirect evidence in human placental cells that ACSL1 and ACSL5 are involved in fatty acid uptake, long-chain PUFAs upregulate both genes and increase fatty acid uptake AA remodeling AAP Likely ACOT1 Q86TX2 641371 Human 9606 Bob Murphy Literature E 16940157 In 16940157, had activity with 16:1, 18:1, 18:1 trans, but was not tested with other unsaturated fatty acids. AAP Likely ACOT2 P49753 10965 Human 9606 Bob Murphy Literature E 16940157 In 16940157, had activity with 16:1, 18:1, 18:1 trans, but was not tested with other unsaturated fatty acids. AAP Gold Standard ACOT4 Q8N9L9 122970 Human 9606 Bob Murphy Literature E 16940157 Activity with 20:4 as substrate in 16940157. AAP Likely ACOT7 O00154 11332 Human 9606 Bob Murphy Literature E 10578051 ACOT7 had very low activity with arachidonyl-CoA, but had greater activity with other long chain acyl-CoAs, including oleoyl-CoA (10578051) AAP Predicted ACOT8 O14734 10005 Human 9606 Bob Murphy Literature E E C E E 10092594 11673457 22465940 9153233 9299485 ACOT8 was ssayed with n-decanoyl-CoA in 10092594. Mouse ACOT8 showed activity with arachidonyl-CoA (11673457), A review article (22465940) suggests that based on broad substrate specificity of mouse and rat ACOT8 it is likely that human ACOT8 also has broad specificity, ACOT8 (AKA hTE) appears to prefer medium chain acyl-CoAs (9153233) with maximal activity for C14-CoA and no activity for C18- and C20-CoA, PMID 9299485 also shows preference for medium chain acyl-CoAs AAP Predicted ACOT11 Q8WXI4 26027 Human 9606 Bob Murphy Literature E 22993230 22897136 AKA BFIT and THEM1 (23700546), mouse Them1 can hydrolyze palmitoyl-CoA (22993230), human BFIT exhibited activity with butyryl- < lauroyl- < myristoyl- < palmitoyl-CoA (22897136) suggesting preference for longer chain substrates, not assayed with arachidonyl-CoA, or unsaturated substrates AAP Gold Standard ACOT13 Q9NPJ3 55856 Human 9606 Bob Murphy Literature E 19170545 In (19170545) enzyme had demonstrated activity with arachidonoyl-CoA AAP Gold Standard BAAT Q14032 570 Human 9606 Bob Murphy Literature E 12810727 In (12810727) enzyme demonstrated thioesterase activity and conjugation to glycine activity for 20:4. AAP Likely GPR17 Q13304 2840 Human 9606 Eicosanoid ligand-binding receptors Reactome: REACT_18352.1 E 16990797 20148890 EC50 values for LTD4 and LTC4 on the order of nM when expressed in 1321N1 and COS-7 cells (16990797). However, 20148890 shows no activation or binding with by LTC4 and LTD4. AAP Gold Standard MGST2 Q99735 4258 Human 9606 PTHR10250 E 8703034 See UniProt entry; catalyzes LTA4 --> LTC4 AAP Gold Standard MGST3 O14880 4259 Human 9606 PTHR10250 E 9278457 AAP Gold Standard OXER1 Q8TDS5 165140 Human 9606 Eicosanoid ligand-binding receptors Reactome: REACT_18352.1 E 12606753 12065583 In 12606753 shown to be a receptor for 5-oxo-ETE (which is formed in 5-LO pathway) and to a lesser extent 5(S)-HpETE. In 12065583, 5-oxo-ETE, 5(S)-HpETE and arachidonic acid are shown to be agonists. AAP Predicted OXGR1 GPR99, (CYSLTR3, but not in humans) Q96P68 27199 Human 9606 Bob Murphy P 23504326 Binding and functional response to LTE4 in mice. AAP Gold Standard PTGR2 Q8N8N7 145482 Human 9606 PI/literature E E 15004468 19000823 15004468 shows human expression pattern and 19000823 shows crystal structure and assays with 15-keto-PGE2, a metabolite of PGE2 AAP Gold Standard ABCC2 MRP2 Q92887 1244 Human 9606 literature C E 20075923 10220572 From review: PMID:10220572 shows transport of LTC(4). Km values reported for ABCC1 and ABCC2 for LTC(4) AAP Gold Standard ABCC3 MRP3, MOAT-D O15438 8714 Human 9606 literature C E 20075923 10987286 From review: PMID: 10987286 shows transport of LTC(4). Km, Vmax reported for LTC4 and other substrates; low - moderate affinity for transport AAP Gold Standard ABCC6 MRP6 O95255 368 Human 9606 literature C E 20075923 12414644 From review: PMID:12414644 shows transport of leukotriene C(4) AAP Likely ABCC10 MRP7 Q5T3U5 89845 Human 9606 literature C E E 20075923 12527806 23087055 From review: PMID: 12527806 shows that LTC(4) is a strong inhibitor of E(2)17betaG transport by ABCC10, but updake of LTC(4) is modest and not observed in all experiments. Ki values for LTC(4) and other compounds; and Km for other substr. In PMID: 23087055, LTC(4) induces ATPase activity. AAP Gold Standard ABCC11 MRP8 Q96J66 85320 Human 9606 literature C E 20075923 15537867 From review: PMID: 15537867 shows transport of leukotriene C(4) AAP Gold Standard AKR1C2 DD; DD2; TDD; BABP; DDH2; HBAB; HAKRD; MCDR2; SRXY8; AKR1C-pseudo P52895 1646 Human 9606 GO E E 8573067 10672042 PMID:8573067 says DD1 and DD2 are probably involved in PG metabolism: DD1 is AKR1C1, DD2 is AKR1C2. In 10672042 kcat and Km with PGD2 and PGE2 as substrates were determined. AAP Predicted ACOX1 Q15067 51 Human 9606 GO P E 7876265 17603022 PMID:7876265 just says that this enzyme oxidizes PG, but it doesn't explore that. In 17603022 only assays up to C18. AAP Gold Standard PNPLA8 IPLA22, IPLA2G Q9NP80 50640 Human 9606 GO E 15695510 PMID:15695510; "results indicate that iPLA2γ has the ability to promote basal and stimulus-coupled AA release; that iPLA2γ displays unique cyclooxygenase (COX) coupling for immediate and delayed PGE2 production.." (couples with COX-1 rather than COX-2 for PGE2 production) AAP Gold Standard AKR1C1 Q04828 1645 Human 9606 literature E E 8573067 10672042 PMID:8573067 says DD1 and DD2 are probably involved in PG metabolism: DD1 is AKR1C1, DD2 is AKR1C2. In 10672042 kcat and Km with PGD2 and PGE2 as substrates were determined. AAP Predicted CYP4B1 P13584 1580 Human 9606 Reactome: REACT_147851.3 Reactome: REACT_13645.1 E E 2574990 10336559 High degree of sequence similarity to rabbit form 5 (2574990). In RABBIT: evidence suggests involvement in metabolizing arachidonic acid to 12-HETE and 12-HETrE (10336559). AAP Predicted CYP8B1 Q9UNU6 1582 Human 9606 Reactome: REACT_147851.3 Reactome: REACT_13645.1 P Evidence: Catalyst Sequence Similarity Project (see Reactome). Could not find publications AAP Predicted DPEP3 Q9H4B8 64180 Human 9606 Reactome: REACT_147851.3 P Could not find publications, also did not find evidence in Reactome. Emailed Reactome (FC) AAP Gold Standard CYP4F12 Q9HCS2 66002 Human 9606 Reactome: REACT_13645.1 GO E 11162607 CYP4F12, expressed in yeast, oxidized arachidonic acid to 18-hydroxyarachidonic acid, and the omega-side chain of two stable prostaglandin (PG) H(2) analogs (11,9-epoxymethano-PGH(2) and 9,11-diazo-15-deoxy-PGH(2)). CYP4F12 oxidized the ω-side chain of leukotriene B4, PGE2, PGF2α, PGH2, and 9,11-epoxymethano-PGH2 poorly AAP Gold Standard AKR1A1 P14550 10327 Human 9606 RGD pathways: PW:0001243 E 23747692 shows in vitro activity in synthesis of PGF2alpha; in vivo relevance not shown (it has only been shown for AKR1B1). Should we add to figure? AAP Gold Standard DAGLB Sn1-specific diacylglycerol lipase beta Q8NCG7 221955 Human 9606 GO E 14610053 PMID: 14610053 shows enzymatic characterization of the human gene AAP Gold Standard DAGLA Sn1-specific diacylglycerol lipase alpha Q9Y4D2 747 Human 9606 GO E 14610053 PMID: 14610053 shows enzymatic characterization of the mouse gene; PMID: 23103940 shows involvement in inflammatory responses in mouse macrophages AAP Gold Standard MGLL Monoglyceride lipase Q99685 11343 Human 9606 GO E 20079333 Overexpressing gene in human non-aggressive cancer cells increased free arachidonic acid (20079333). AAP Predicted GGTLC1 Gamma-glutamyltransferase light chain 1 Q9BX51 92086 Human 9606 GO P 18357469 There is expression data but no protein data. AAP Gold Standard SLCO2B1 Solute carrier organic anion transporter family member 2B1 O94956 11309 Human 9606 GO E 10873595 Uptake of [3H]prostaglandin E2 by HEK293 cell transfected with gene (10873595). AAP Predicted AOPEP Aminopeptidase O Q8N6M6 84909 Human 9606 GO P 15687497 Structural similarity to leukotriene A4 hydrolase AAP Predicted RNPEP Aminopeptidase B Q9H4A4 6051 Human 9606 GO P E 12119107 10215585 The human and mouse enzymes show strong structural relationships with mammals LTA4 hydrolases. 10215585 found no epoxide hydrolase activity (LTA4->LTB4). AAP Predicted RNPEPL1 Arginyl aminopeptidase-like 1 Q9HAU8 57140 Human 9606 GO E 19508204 only found one publication: 19508204. States "...and was insensitive to specific inhibitors of the closely-related arginyl aminopeptidase, indicating similarity to leukotriene A(4) hydrolase" AAP Gold Standard SLCO2A1 Solute carrier organic anion transporter family member 2A1 Q92959 6578 Human 9606 GO E 8787677 When transiently expressed in HeLa cells, a full-length clone catalyzed the transport of PGE1, PGE2, PGD2, PGF2alpha, and, to a lesser degree, TXB2